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The restriction sites were introduced by PCR using the primer pairs 5-TGGATCCGCTCAAGAGTTTGTGAACTG-3 and 5-CTAAGCTTTTACTTGTACAGCTCGTCC-3 for GRX1-roGFP2iL and 5-AAGGATCCGTGAGCAAGGGCGAGGAGC-3 and 5-CTAAGCTTTTACTTGTACAGCTCGTCC-3 for roGFP2-iL

Cell migration assay To analyze the cell migration ability, HepG2 cells were transfected with siRNAs and SNU449 cells were transfected with overexpression plasmids for 48 h

The supernatant was discarded, and isolated cells were separated from debris/myelin using a 70%/37%/30% layered Percoll gradient (Sigma-Aldrich, P1644) with centrifugation at 300 g for 40 min at 18 C without braking

doi:10.1016/S0022-2836(05)80181-0 [PubMed] [Elsevier] 110